仔猪不同发育阶段结肠微生物菌群特征分析

doi:10.11843/j.issn.0366-6964.2019.09.004

Abstract

Abstract: The objective of this study was to compare the structure and composition of colon microflora of Mashen and Jinfen White pigs at different developmental stages using Illumina MiSeq sequencing technology. In present study, Mashen and Jinfen White pigs were used as experimental animals. Three boar piglets with similar weight were randomly selected at the stage of 1, 28 and 70 days old, respectively, and the colon microbial diversity was analyzed using 16S rRNA high-throughput sequencing technology. The intestinal microorganisms of piglets in two breeds were found to be distributed in 15 phyla, 28 classes, 59 orders, 100 families and 290 genus by bacteria taxonomy analysis. The Firmicutes and Bacteroides are the dominant bacteria, accounting for 49.03% and 31.94% respectively. There was no significant difference in intestinal microbial diversity of piglets at birth between breeds. The microbial diversity increased significantly with the growth and development of piglets, and basically stabilized at the stage of Jinfen White piglet conservation (28-70 d). The specific flora of two breeds of piglets at different stages showed significant difference with the increase of age. Association analysis showed that LPS, TNF-α, IL-6 were significantly correlated with intestinal flora (P<0.05), while the DAO and D-LA had no significant correlation with intestinal flora (P>0.05). The colonic microflora correlated with LPS,TNF-α,IL-6 was different between the two breeds. It is concluded that the structure and composition of colon microflora of piglets were significantly different between two breeds and among different developmental stages,which play the important roles in immune function of piglets.

CLC Number:

S811.6

GUO Xiaohong, GUO Yulong, LIU Yadan, LIU Juan, SHI Wenqian, DONG Lei, CAI Chunbo, CAO Guoqing, LI Bugao, GAO Pengfei. Characteristics of Intestinal Microflora of Colon at Different Developmental Stages in Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1759-1774.

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Characteristics of Intestinal Microflora of Colon at Different Developmental Stages in Piglets

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